Articles by the group in 2000

The ionization of a buried glutamic acid is thermodynamically linked to the stability of Leishmania mexicana triose phosphate isomerase.
Lambeir AM, Backmann J, Ruiz-Sanz J, Filimonov V, Nielsen JE, Kursula I, Norledge BV, Wierenga RK
EJB (2000) 267: 2516-2524.

PubMed Abstract

Synergism with the Coactivator OBF-1 (OCA-B, BOB-1) Is Mediated by a Specific POU Dimer Configuration.
Tomilin A, Remenyi A, Lins K, Bak H, Leidel S, Vriend G, Wilmanns M, Scholer HR.
Cell. (2000) 103: 853-864.

PubMed Abstract

G protein-coupled receptors, or the power of data
F. Horn, M. Mokrane, J. Weare, and G. Vriend, In
Genomics and Proteomics: Functional and Computational Aspects Ed. S. Suhai., Kluwer Academic / Plenum publishers, New York, (2000) pp 191-214.

Everything, and a little bit more, you ever wanted to know (but were too afraid to ask) about the collection and harvesting of GPCR data.

Receptors coupling to G proteins: is there a signal behind the sequence?
Horn F, Van der Wenden EM, Oliveira L, IJzerman AP, Vriend G.
Proteins (2000) 41: 448-59.

Medline abstract

Amyloids protect the silkmoth oocyte and embryo.
Iconomidou VA, Vriend G, Hamodrakas SJ.
FEBS Lett. (2000) 479: 141-145.

The most under-rated article I have ever been involved in. Thanks to the brilliant experimental work of my co-authors (Stavros and Bessie), hard evidence was provided that the shell around the silkmoth oocyte (similar to that tasteless layer of irregularly shaped half-hard white stuff between the hard shell and the nice part of your boiled egg on sunday morning) is amyloidal. On top of that, we managed to show that this is a 'normal' amyloid with the same characteristics as those that cause havoc in the brain of people who believed their goverments and ate too much beef.
Full text.

Protein engineering of bacterial alpha-amylases.
Nielsen JE, Borchert TV.
Biochim Biophys Acta. (2000) 1543, 253-274.